Additional Duties of Phlebotomist

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27 April 2016

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-Urine specimen obtained after a thorough cleansing of the glans penis (males) and the labia and urethral meatus (females). -Following the cleansing procedure, the patient passes the first portion of urine into the toilet, stops and collects the mid-portion in the specimen container. -This provides a sample that is less contaminated by epithelial cells and bacteria (normal flora). Methods of Urine Collection

-Specimen of choice for patient suffering from urinary retention. (Patient cannot void voluntarily) -The specimen is collected under sterile conditions by passing the catheter through the urethra into the bladder. -The specimen is used for BACTERIAL CULTURE.

-If routine urinalysis is requested, culture should be performed first to prevent contamination.

-Involves urine collection directly from the bladder by puncturing the abdominal wall and the bladder using a needle syringe. -Since the bladder is normally sterile, it is primarily used for bacterial cultures and for infants to whom contamination is unavoidable. -The only specimen acceptable for both aerobic and anaerobic urine culture. -Can be used for cytological analysis of urine

Urine Culture
-Samples collected by MSCC, Catheterized & Suprapubic aspiration can be used for AEROBIC CULTURE. -The only urine collection method acceptable for ANAEROBIC CULTURE is SUPRAPUBIC ASPIRATION. -COMMERCIALLY available plastic urine collection bags with hypoallergenic skin adhesive. -Also referred as wee bag.

Uses chain of custody (COC) which refers to the process that provides documentation of proper sample identification from the time of collection to the receipt of laboratory results. The COC is a standardized form that must document and accompany every step of drug testing, from collector to courier to laboratory to medical review officer to employer.


-Required volume of urine 30-45 ml
-The urine temperature must be taken within 4 minutes from the time of collection to confirm the specimen has not been adulterated. -The temperature should read within the range of 32.5C to 37.7C. -Recollection of a second specimen as soon as possible will be necessary in the following cases: Urine temperature outside the recommended range

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