Cell bio lab report

Purpose:

During this experiment we compared the hemagglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample, and determine through observations whether or not addition of galactose or mannose will inhibit this reaction. I hypothesize that the Con A + galactose solutions will have partial agglutination and partial no agglutination, and the Con A + mannose solutions will have all no agglutination.

Results:

Rows A and B had half agglutination and half no agglutination, while row C had all no agglutination. Row D had half agglutination and half partial agglutination, while row E had 4 columns with agglutination and 8 columns with no agglutination. Row F had complete agglutination throughout. Con A reaction plate (Row/Column)

Reaction
A1-A6 (Control)
Agglutination/inhibited
A7-A12 (Control)
No Agglutination/not inhibited
B1-B6 (Con A + galactose)
Agglutination/inhibited
B7-B12 (Con A + galactose)
No Agglutination/not inhibited
C1-C12 (Con A + mannose)
No Agglutination/not inhibited
D1-D5 (Sample)
Agglutination/inhibited
D6-D12 (Sample)
Partial agglutination/inhibited
E1-E4 (Con A + galactose)
Agglutination/inhibited
E5-E12 (Con A + galactose)
No Agglutination/not inhibited
F1-F12 (Con A + mannose)
No agglutination/not inhibited
G1-G12 ((-)Control)
Partial agglutination/inhibited
H1-H12 (RBCs)
Partial agglutination/inhibited

Discussion:

My hypothesis was proven correct, the Con A + galactose solutions did have partial agglutination and partial no agglutination, and the Con A + mannose solutions did have all no agglutination. This means that in the Con A + galactose solutions the ones that had agglutination the red cells aggregated with the lectin and sediment in the form of a uniform layer that covered the whole bottom of the well including the slopes, and in the Con A + mannose solutions the ones that failed to have agglutination take place covered only part of the bottom. We found through this experiment that the control Con A sample and the Con A sample had very similar strengths of reaction.

Conclusion:

In this experiment through serial dilutions on a 96 well plate we determined the strength of the each reaction on both the Con A sample and the control sample. We found through our experiment that the addition of galactose or mannose will partially inhibit or inhibit the reaction between our Con A sample and control Con A sample.